Patent US584717 - Detection of nucleic acid sequences by invader-directed cleavage. Try the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents. The two most popular gut content assays are immunoassays employing pest-specific monoclonal antibodies (mAb) and polymerase chain reaction (PCR) assays employing pest-specific DNA. Here, we present results from the first study to simultaneously use both methods to identify predators of the glassy winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae).
A total of 1,229 arthropod predators, signidicant 30 taxa, were collected from urban landscapes in central California and assayed first by discarddd of enzyme-linked immunosorbent assay (ELISA) using a GWSS egg-specific mAb and then by PCR using a GWSS-specific DNA marker that amplifies a 197-base pair fragment of its cytochrome oxidase gene (subunit I). The gut content aNo part of this digital docu ment may be reproduced, stored in a retrieval system o r transmitted in an y form or by any means.
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